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Tissue Antigens 67 3: All the authors have approved the manuscript for publication. J Leukoc Biol 3: Hum Immunol 77 Due to the time-consuming nature of manual KIR allele assignment, two different algorithms are needed to ensure the reliability of NGS-based typing methods for the identification of KIR allele polymorphisms. Immunology 3: Our results demonstrated successful long-range KIR gene amplifications of all reference samples using intergenic KIR primers. Table 2 Discordant killer cell immunoglobulin-like receptor KIR typing of International Histocompatibility Workshop samples observed between legranv obtained by exome capture a 47 and those obtained by next generation sequencing NGS in this study.
Hum Immunol 65 6: The final two digits are used to distinguish alleles that only differ by substitutions in an intron, promoter, or other non-coding region of the sequence. Exome capture from saliva produces high quality genomic and metagenomic data.
Items in search results. Reads were mapped to the human genome reference sequence hg19 using the Burrows—Wheeler Aligner Memory Efficient Mapping tool. Immunity 15 3: For KIR allele assignment, a manual bioinformatic pipeline was first used in the absence of available softwares. Investigation of killer cell immunoglobulin-like receptor gene diversity III. In this step, calculation for depth legdand each position and the number of sequences used for each allele are determined.
Relevance of KIR gene matching in unrelated hematopoietic stem cell transplantations. Format see all Format.
Refine your search for legrand. Discrimination between the main activating and inhibitory killer cell immunoglobulin-like receptor positive natural killer cell subsets using newly characterized monoclonal antibodies. KG designed the study, analyzed data, and wrote the paper. J Immunol Format see all Format. Our study evaluated the performance of different algorithms for KIR allele assignment.
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Other Collectable Items Received Jan 23; Accepted Apr Each bar represents one specific centromeric or telomeric KIR gene. Other Books, Comics, Magazines The pooled and barcoded libraries were denaturated with 0.
Number of bids and bid amounts may be slightly out of date. The authors would also like to thank Dr. Coverage mean is calculated on each gene position, and a threshold of 10 is applied in order to ascertain its absence or presence. Human diversity in killer cell inhibitory receptor genes. Investigation of killer cell immunoglobulinlike receptor gene diversity: Custom Bundle see all Custom Bundle.
Tissue Antigens 64 3: Show only see all Show only. All listings filter applied.
Refine more Format Format. Our method of typing all KIR genes at high resolution provides an alternative, easily implemented method practice, to study the KIR allele polymorphisms.
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Third, the data file from Blast was used to merge all sequences together to construct each allele. Alleles and intron polymorphism of KIR3DL1 shown by combination of allele group-specific primers and sequencing. Non-Sport Trading Cards In this legranx, the depth for each position and the number of sequences legrabd each allele were calculated. It is likely that this is due to the fact that only coding regions CDS were taken into account for allele variation comparison.